Endpoint PCR

High Fidelity PCR and Cloning

Reverse Transcription

Quantitative PCR

Endpoint PCR Green Line

Taq Polymerase GL

2X Master Mix GL

dNTPs and dNTPs mix

Gel Loading Buffer GL

Agarose LE

DNA Ladders GL

2X XtraWhite Master Mix and 2X XtraRTL Master Mix (GeneSpin proprietary formulation), are two premixed, ready-to-use solution containing Xtra-Taq Pol, dNTPs and MgCl2 in a Reaction Buffer optimized for use in PCR amplification of targets present in low copy number and to avoid amplification of non-specific products. Both buffers contain 3.0mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use). 2X Master Mix Standard GL contain an internal fluorescent stain for DNA detection on Agarose gel directly after PCR amplification.

2X XtraRTL Master Mix contains Orange G dye that allows gel loading and electrophoresis of the sample directly from the PCR tube, without further manipulation. 2X XtraWhite Master Mix is supplied with appropriate quantity of 6X Loading Dye. The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.

*2X XtraWhite Master Mix GL is supplied with appropriate quantity of 6X Loading Dye

DNA Template

Assay Set-Up:

Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

Cycling conditions:

Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.