cat. no.

amount

note

STS-MICOP 25

250ul

25 assay kit

1. Transfer 100-200ul of cell culture supernatant into 1.5ml centrifuge tube. Incubate the supernatant at 95°C for 5 minutes.

2. Centrifuge at maximum speed for 5 minutes.

3.Use 2-5ul of the supernatant as PCR template.

IMPORTANT! Before harvesting the supernatant from the cell culture, cells should cover approximately 90% of the growth surface! The supernatant may cause PCR inhibition in excessively dense cell cultures (>90%).

4. For optimum separation we recommend using a 2% agarose gel with TAE or TBE buffer used for electrophoresis.

5. Load all PCR product volume (20ul) directly onto the gel and perform electrophoresis.

6. When the electrophoretic run is completed, lay the gel onto UV transilluminator to detect the expected band.

7. IF the test is POSITIVE, a DNA band of 750bp will appear.

component

test sample

test sample

MG water

8-5ul

   8ul

denaturation

95°C

5min

denaturation (1)

95°C

60 sec

final elong.

72°C

5 min

final step

4°C

forever

yours

MOLECULAR BIOLOGY

reagents

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