Pfu DNA Pol is a superior thermostable DNA Pol isolated from an E.coli strain carrying the “pol” gene from Pyrococcus furiosus with 5’ →3’ polymerase activity and 3’ → 5’ exonuclease proofreading activity, i.e. it catalyzes DNA synthesis with very low error rate (about twelve times more accurate than Taq Pol). However, Pfu DNA Pol is slower than Taq Pol and typically requires about 1-2 min per cycle to amplify 1Kb of DNA.Using Pfu DNA Pol in PCR reactions also results in blunt-ended amplification products. Pfu DNA Pol is hence superior for techniques that require high fidelity DNA synthesis, but can also be used in conjunction with Taq Pol to obtain the fidelity of Pfu with the speed of Taq Pol activity.

Pfu DNA Polymerase is supplied with 5X XtraWhite (GeneSpin proprietary formulation) developed for standard and/or high-fidelity amplification of high-GC (>75%) templates.

Ligase

T4 DNA Ligase

T4 DNA Ligase (isolated and purified from an E. coli strain) catalyzes the formation of a phosphodiester bond between juxtaposed5’-phosphate and 3’-hydroxyl groups in duplex DNA or RNA. This enzyme is capable of joining adjacent nucleotides in either a blunt-ended or cohesive ended configuration, as well as repairing single-stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.