MMLV Mix 5X full (with or without RNase Inhibitor) is a premixed, ready-to-use reagent (GeneSpin proprietary formulation) containing, dNTPs, MgCl2 , DDT, oligo d(T), random primers RNase Inhibitor and stabilizers optimized for first strand cDNA synthesis. M-MLV Reverse Transcriptase is supplied directly in the SuperMix 5X. RNase inhibitor is supplied directly in the SuperMix 5X. The MMLV Mix 5X full composition is particularly recommended for gene expression analysis in RT-PCR and Real time RT-qPCR.
cat. no.
cat. no.
amount
note
cat. no.
amount
note
100 ul
4x100 ul
M-MLV in the same tube with RNase
M-MLV in the same tube with RNase
STS-MSXFw 10
STS-MSXFw 100
100 ul
4x100 ul
M-MLV in the same tube w/o RNase
M-MLV in the same tube w/o RNase
FOR RESEARCH USE ONLY
SHIPPING
Shipped in green ice.
CONCENTRATION
5X M-MLV Super Mix Full
FORM
Liquid
SHELF LIFE
12 months
STORAGE
Store at -20°C
Components
component
STS-MSX F 10
STS-MSX F 100
STS-MSXFw 10
STS-MSXFw 100
5X M-MLV Buffer FULL
RNase free water
RNase
100 ul/ 25 reaction
1 ml
In the 5X M-MLV buffer
4x100 ul/ 100 reactions
1ml
In the 5X M-MLV buffer
100 ul/ 25 reaction
1 ml
-
4x100 ul/ 100 reactions
1ml
-
Assay set up
Before starting, vortex all components thoroughly to ensure homogeneity.
Prepare a premix for the number of assays you need according to the following protocol:
component
stock conc.
final conc.
20ul reaction
5X M-MLV Buffer
RNA
5X
-
1X
-
4.0ul
1ng to 4ng
RNase free water
-
-
up to20ul
First cDNA synthesis
Spin down the tubes/plate briefly to remove bubbles.
25°C
5min
42°C
30 min
85°C
2 min
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