Products


Molecular Biology Reagents


END point PCR green line

Circle Check

2X Master Mix Standard White and RTL GL

2X XtraWhite Master Mix GL and 2X XtraRTL Master Mix GL  (GeneSpin proprietary formulation), are two premixed, ready-to-use solution containing Xtra-Taq Pol, dNTPs and MgCl2 in a Reaction Buffer optimized for use in PCR amplification of targets present in low copy number and to avoid amplification of non-specific products. Both buffers contain 3.0mM magnesium, PCR enhancers and thickening agents (vortex thoroughly prior to use). 2X Master Mix Standard GL contain an internal fluorescent stain for DNA detection on Agarose gel directly after PCR amplification.

2X XtraRTL Master Mix GL contains Orange G dye that allows gel loading and electrophoresis of the sample directly from the PCR tube, without further manipulation. 2X XtraWhite Master Mix is supplied with appropriate quantity of 6X Loading Dye. The Orange G dye migrates at the same rate as a duplex DNA fragment of approximately 50 Kbp and does not interfere with DNA migration when it is used as a loading dye for agarose gel electrophoresis.

GREEN LINE OVERVIEW

2X Master Mix GL contains an internal fluorescent stain for DNA detection on Agarose gel directly after PCR amplification.

cat. no.

amount

note

cat. no.

amount

note

STS-XMMixW 200 GL*

STS-XMMixW 1000 GL*

PDF

5 ml

25 ml

*2X XtraWhite Master Mix is supplied with appropriate quantity of 6X Loading Dye

2X Xtra White Master Mix GL

2X Xtra White Master Mix GL

STS-XMMixRTL 200 GL

STS-XMMixRTL 1000 GL

PDF

5 ml

25 ml

2X Xtra RTL Master Mix GL

2X Xtra RTL Master Mix GL

FOR RESEARCH USE ONLY

SHIPPING

Shipped in green ice.

FORM

Liquid

SHELF LIFE

12 months

UNIT DEFINITION

One unit is defined as the amount of enzyme required to incorporate 10 nanomoles of dNTPs into acid-insolubile material in 30 min at 74°C.

CONCENTRATION

2X conc.

STORAGE

Store at -20°C

Assay set up


Before starting, vortex all components thoroughly to ensure homogeneity.

Prepare a premix for the number of assays you need according to the following protocol:

component

stock conc.

final conc.

30ul reaction

2X Master Mix GL

primers

DNA Template

2X

-

1ug/ul each

1X

50ng/ul each

-

3.0ul

2.0ul each

10-20ng

MG Water

-

-

up to 30ul

Cycling conditions

Cycling conditions:

Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.

denaturation

95°C

5min

denaturation (1)

95°C

30 sec

annealing (2)

extension

50-68°C

72°C

30 sec

30 sec

final elong.

72°C

5 min

final step

4°C

forever

1)The annealing temperature depends on the melting temperature of the primers used.

2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

Technical Support

Contact us at info@genespin.com to request Technical support with subject and details of your question/inquiry

Products

Molecular Biology

Proteins 

Research

Buffers

Reagents

yours

MOLECULAR BIOLOGY

reagents

Growth

Media

Antibiotics

Cells culture

Transfection

Inhibitors

© Copyright 2025 GeneSpin Srl. All rights reserved.

Questo sito web fa uso di cookies. Si prega di consultare la nostra informativa sulla privacy per i dettagli.

Non consentire

OK