cat. no.
amount
note
QSTS-OLRSMMix 200 RTL
QSTS-OLRSMMix 500 RTL
5 ml
12.5 ml
2X blue + 1.5ml 40X Yellow - low ROX
2X blue + 4.0ml 40X Yellow - low ROX
QSTS-OLRSMMix 1000 RTL
5x5 ml
2X blue + 8.0ml 40X Yellow - low ROX
FOR RESEARCH USE ONLY
SHIPPING
Shipped in green ice.
FORM
Liquid
SHELF LIFE
12 months
STORAGE
Store at -20°C
CONCENTRATION
2X conc.
Assay set up
Before starting, vortex all components thoroughly to ensure homogeneity.
Prepare a premix for the number of assays you need according to the following protocol:
component
stock conc.
final conc.
20ul reaction
2X Master Mix
primer for
primer rev
DNA Template
2X
-
10 uM
10 uM
1X
100-400 nM
100-400 nM
-
10.0 ul
0.2-0.8 ul
0.2-0.8 ul
< 500ng
MG Water
-
-
up to 20ul
Cycling conditions
50ul reaction
25.0 ul
0.5-2.0 ul
0.5-2.0 ul
< 500ng
up to 50ul
*use 40X Yellow for diluition
REMEBER: The green color indicates that
the yellow sample A has been added into blue qPCR Master Mix
TEMPLATE and 40X Yellow assay set-up:
IN A FINAL qPCR REACTION OF 20ul
Master Mix and Primers and water
DNA Template**
15ul
5ul
**Mix 40X Yellow at final conc. 1X and 5ul of DNA Template. Use 5ul of the mix
Cycling conditions:
Spin down the tubes/plate briefly to remove bubbles and place them into the cycler.
denaturation
95°C
5min
denaturation (1)
95°C
30 sec
annealing (2)
and extension
55-68°C
30 sec
melt curve
from 65°C
to 95°C
0.5°C/5sec
1)The annealing temperature depends on the melting temperature of the primers used.
2)The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.
SYBR® is a registered trademark of Invitrogen Corporation, Carlsbad, California, USA
